Mondal Kalyan K, Chatterjee Subhas Chandra, Viswakarma Navin, Bhattacharya Ram Charan, Grover Anita
Chaudhary Swaran Kumar Himachal Pradesh Agricultural University, Research Station, Sangla Valley, Kuppa (Zira Farm), Kinnaur-172 106, Himachal Pradesh, India.
Curr Microbiol. 2003 Sep;47(3):171-3. doi: 10.1007/s00284-002-3980-6.
Chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. In the present study, Brassica plant (Brassica juncea L.) was transformed with chitinase gene tagged with an overexpressing promoter 35 S CaMV. The putative transgenics were assayed for their inhibitory activity against Alternaria brassicae, the inducer of Alternaria leaf spot of Brassica both in vitro and under polyhouse conditions. In in vitro fungal growth inhibition assays, chitinase inhibited the fungal colony size by 12-56% over the non-trangenic control. The bioassay under artificial epiphytotic conditions revealed the delay in the onset of disease as well as reduced lesion number and size in 35S-chitinase Brassica as compared to the untransformed control plants.
几丁质酶能够降解入侵的植物致病真菌的细胞壁，在植物防御反应中发挥重要作用，特别是当这种酶通过基因工程过度表达时。在本研究中，用带有过表达启动子35S CaMV的几丁质酶基因转化了芸苔属植物（芥菜）。对推定的转基因植株在体外和温室条件下针对芸苔链格孢（芥菜链格孢叶斑病的病原体）的抑制活性进行了测定。在体外真菌生长抑制试验中，与非转基因对照相比，几丁质酶使真菌菌落大小减小了12%-56%。人工流行条件下的生物测定表明，与未转化的对照植株相比，35S-几丁质酶转基因芥菜发病时间延迟，病斑数量和大小减少。
