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[使用部分改良标准培养基对海水中大肠杆菌随时间变化后的检测进行比较研究]

[Comparative study of the detection of E. coli after time in seawater with partially modified standard media].

作者信息

Kolbeck K, Richter K D, Heinemeyer E A

机构信息

Staatliches Medizinaluntersuchungsamt Aurich.

出版信息

Zentralbl Hyg Umweltmed. 1992 Oct;193(3):224-9.

PMID:1457033
Abstract

Fecal coliform bacteria (E. coli) are the most important parameter when monitoring the hygienic quality of bathing water according to EC guidelines. Among other things the number of E. coli is clearly dependent on the length of exposure of the bacteria to marine water as well as the media used to establish the number. In a seven-day salt water stress experiment the best rate of recultivation was achieved on average with DEV lactose peptone broth. The Brila-MUG medium produced a slightly lower rate of recultivation and the fluorocult-lauryl-sulfate-broth the lowest rate of recultivation. After a shorter exposure of bacteria to marine water conditions (up to 24 h) the DEV-lactose-broth produced slightly lower rates of recultivation. An alteration of the standard components of the media did not lead to an improvement in the rate of recultivation.

摘要

根据欧盟委员会的指导方针,粪大肠菌群(大肠杆菌)是监测沐浴水卫生质量时最重要的参数。除其他因素外,大肠杆菌的数量显然取决于细菌暴露于海水的时长以及用于确定数量的培养基。在一项为期七天的盐水胁迫实验中,使用DEV乳糖蛋白胨肉汤平均实现了最佳的再培养率。Brila-MUG培养基的再培养率略低,而氟培养-月桂基硫酸盐肉汤的再培养率最低。在细菌较短时间暴露于海水条件下(长达24小时),DEV乳糖肉汤的再培养率略低。培养基标准成分的改变并未导致再培养率的提高。

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