Neidhardt S, Havemeister G, Höller C, Gundermann K O
Institut für Hygiene und Umweltmedizin, Christian-Albrechts-Universität Kiel.
Zentralbl Hyg Umweltmed. 1995 Dec;198(2):152-64.
Four media containing 4-methylumbelliferyl-beta-D-glucuronide were evaluated as a non-confirmatory procedure for E. coli detection in recreational water surveillance. The media included ECD-Agar for membrane filtration and laurylsulphate-tryptose, brilliant-green-bile and lactose as broth media in a three tube most probable number procedure. From six representative water sites, samples were collected weekly over a typical summer season (17.05-27.09.1994) and processed as parallels, using each media at two different incubation temperatures (36 degrees/44 degrees C). Results showed that incubation temperature had no impact on E. coli counts. Each media at a given temperature could be regarded as individual enrichment procedure. None of these enrichment procedures showed a constant and predictable higher sensitivity during the sampling period at all sites compared to the others tested. For parallel results, the rate of agreement, based upon EC-guideline (76/160/EWG) staging of recreational water quality, was 85% for membrane filtration and 75% for the MPN-procedure results. Marked differences could be observed in false-positive specificity showing correlation to the selective characteristics of the media. Subsequently lactose-broth at 44 degrees C performed worst with 30% non verifiable results, while ECD-agar and laurysulphate-tryptose-broth, both at 44 degrees C, had a nearly 100% confirmation rate. Thus, combining high specificity with no lack in sensitivity these two MUG-supplemented media seem to be best suited for E. coli detection in routine recreational water surveillance.
对含有4-甲基伞形酮基-β-D-葡萄糖醛酸的四种培养基进行了评估，作为休闲水监测中大肠杆菌检测的一种非确证性方法。这些培养基包括用于膜过滤的ECD琼脂，以及在三管最大可能数法中作为肉汤培养基的月桂基硫酸盐胰蛋白胨、亮绿胆汁和乳糖。在一个典型的夏季（1994年5月17日至9月27日），从六个代表性水域每周采集样本，并作为平行样本进行处理，在两种不同的培养温度（36℃/44℃）下使用每种培养基。结果表明，培养温度对大肠杆菌计数没有影响。在给定温度下的每种培养基都可视为单独的富集方法。在所有采样点的采样期间，与其他测试方法相比，这些富集方法均未显示出始终如一且可预测的更高灵敏度。对于平行结果，根据欧盟委员会（76/160/EWG）休闲水质量分级标准，膜过滤法的一致性率为85%，最大可能数法的结果为75%。在假阳性特异性方面可观察到明显差异，这与培养基的选择性特征相关。随后，44℃下的乳糖肉汤表现最差，不可验证结果为30%，而44℃下的ECD琼脂和月桂基硫酸盐胰蛋白胨肉汤的确认率接近100%。因此，这两种添加MUG的培养基兼具高特异性且不缺乏灵敏度，似乎最适合在常规休闲水监测中检测大肠杆菌。
