Mei Erwen, Vinogradov Sergei, Hochstrasser Robin M
Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
J Am Chem Soc. 2003 Oct 29;125(43):13198-204. doi: 10.1021/ja030271k.
Single molecules are detected through the phosphorescence emission of their triplet states. Emission of the triplet states of single molecules of Pt octabutoxycarbonyl porphyrin (PtOBCP) and ruthenium(II)-tris-4,7-diphenyl-1,10-phenanthroline dichloride (Ru(dpp)(3)Cl(2)) is reported. The single molecule phosphorescence is very sensitive to molecular oxygen. Each molecule has its own characteristic quenching rate by oxygen, and the distribution of these rates is measured for (Ru(dpp)(3)Cl(2)) on a quartz surface. The large variance of this distribution is presumed to be caused by fluctuations in the pseudobimolecular rate coefficient and the local oxygen concentration. The possibility of creating a quantitative single oxygen molecule sensor is suggested.
通过单分子三重态的磷光发射来检测单分子。报道了八丁氧基羰基卟啉铂（PtOBCP）和二氯化钌（II）-三-4,7-二苯基-1,10-菲咯啉（Ru(dpp)(3)Cl(2)）单分子的三重态发射。单分子磷光对分子氧非常敏感。每个分子都有其自身由氧引起的特征猝灭速率，并且在石英表面上测量了（Ru(dpp)(3)Cl(2)）的这些速率分布。据推测，这种分布的较大方差是由假双分子速率系数和局部氧浓度的波动引起的。提出了创建定量单氧分子传感器的可能性。
