Motshwene Precious, Karreman Robert, Kgari Gail, Brandt Wolf, Lindsey George
Department of Molecular and Cellular Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Biochem J. 2004 Feb 1;377(Pt 3):769-74. doi: 10.1042/BJ20031301.
Yeast cells Saccharomyces cerevisiae, late embryogenic abundant-like stress response protein Hsp 12 (heat-shock protein 12) were found by immunocytochemistry to be located both in the cytoplasm and in the cell wall, from where they could be extracted with dilute NaOH solutions. Yeast cells with the Hsp 12 gene disrupted were unable to grow in the presence of either 12 mM caffeine or 0.43 mM Congo Red, molecules known to affect cell-wall integrity. The volume of yeast cells were less affected by rapid changes in the osmolality of the growth medium when compared with the wild-type yeast cells, suggesting a role for Hsp 12 in the flexibility of the cell wall. This was also suggested by subjecting the yeast cells to rapid changes in barometric pressure where it was found that wild-type yeast cells were more resistant to cellular breakage.
通过免疫细胞化学发现，酿酒酵母中的酵母细胞、晚期胚胎丰富样应激反应蛋白热休克蛋白12（Hsp 12）位于细胞质和细胞壁中，可用稀氢氧化钠溶液从这些部位提取。Hsp 12基因被破坏的酵母细胞在12 mM咖啡因或0.43 mM刚果红（已知会影响细胞壁完整性的分子）存在的情况下无法生长。与野生型酵母细胞相比，酵母细胞的体积受生长培养基渗透压快速变化的影响较小，这表明Hsp 12在细胞壁的柔韧性中发挥作用。对酵母细胞进行气压快速变化实验也表明了这一点，实验发现野生型酵母细胞对细胞破裂更具抵抗力。
