Ebel F, Schmitt E, Peter-Katalinić J, Kniep B, Mühlradt P F
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, FRG.
Biochemistry. 1992 Dec 8;31(48):12190-7. doi: 10.1021/bi00163a031.
On the basis of the pattern of lymphokines they secrete, murine T helper clones can be divided into two subsets, TH1 and TH2. This concept of two different T helper effector cells helps to explain the diversity of immune reactions occurring in different parts of the body. The in vivo localization of T helper subtypes is of great interest, but up to now no biochemical or surface markers were available to distinguish between them. We analyzed the glycolipids from altogether 12 murine TH1 and TH2 cell lines or clones. A comparison of the gangliosides by thin-layer chromatography showed differences between the TH1 and TH2 cells. Binding studies with specific antibodies to asialo backbone structures after degradation by neuraminidases showed that the main gangliosides from these lymphocytes shared a common GgOse4 backbone and thus differed only in their degree or position of sialylation. Two disialogangliosides appeared to be characteristic. They were isolated from the D10.G4.1 TH2 cell clone and identified by fast atom bombardment mass spectrometry as IVNeuAc,IINeuAc-GgOse4Cer (GD1a) and IVNeuAc,IIINeuAc-GgOse4Cer (GD1 alpha), respectively. GD1a was characteristically only detected in TH2 cells, whereas GD1 alpha was preferably, but not exclusively, expressed by TH1 lymphocytes. Although GD1a was also found in the lung, heart, kidney, and spleen, its expression within the murine immune cells under investigation was unique to TH2 lymphocytes. Scarcely any GD1a was found in thymocytes, B cells, or CD8 positive (cytolytic) T cells, but significant expression was seen in CD4 positive (helper) T cells which include the TH2 subpopulation.(ABSTRACT TRUNCATED AT 250 WORDS)
根据小鼠T辅助细胞克隆分泌的淋巴因子模式，可将其分为两个亚群，即TH1和TH2。这两种不同的T辅助效应细胞的概念有助于解释身体不同部位发生的免疫反应的多样性。T辅助亚群在体内的定位备受关注，但到目前为止，尚无生化或表面标志物可用于区分它们。我们分析了总共12种小鼠TH1和TH2细胞系或克隆的糖脂。通过薄层色谱法对神经节苷脂进行比较，结果显示TH1和TH2细胞之间存在差异。用神经氨酸酶降解后针对去唾液酸骨架结构的特异性抗体进行结合研究表明，这些淋巴细胞的主要神经节苷脂具有共同的GgOse4骨架，因此仅在唾液酸化程度或位置上有所不同。两种二唾液酸神经节苷脂似乎具有特征性。它们从D10.G4.1 TH2细胞克隆中分离出来，并通过快原子轰击质谱法分别鉴定为IVNeuAc、IINeuAc-GgOse4Cer（GD1a）和IVNeuAc、IIINeuAc-GgOse4Cer（GD1α）。GD1a的特征是仅在TH2细胞中检测到，而GD1α则主要（但并非唯一）由TH1淋巴细胞表达。尽管在肺、心脏、肾脏和脾脏中也发现了GD1a，但其在所研究的小鼠免疫细胞中的表达是TH2淋巴细胞所特有的。在胸腺细胞、B细胞或CD8阳性（细胞溶解）T细胞中几乎未发现GD1a，但在包括TH2亚群的CD4阳性（辅助）T细胞中观察到明显表达。（摘要截选至250词）
