Li Jing, Jia Jing Fen, Hao Jian Guo
School of Life Science, Northwest University, Xi'an 710069.
Shi Yan Sheng Wu Xue Bao. 2003 Aug;36(4):318-21.
The cotyledonary segments of sterile seedlings of Helianthemum Songaricum Schrenk were cultured on different media containing different phytohormons. It was found that the calli could be induced efficiently on MS basal medium supplemented with 10.0 mg/L NAA and 0.2 mg/L 6-BA. When calli were transferred on MS medium with 2.0 mg/L 6-BA and 0.2 mg/L NAA, shoots were produced. The frequency of shoot differentiation reached about 85%. The regenerated shoots were rooted on 1/2MS medium added with 0.5 mg/L NAA. The rooting rate was about 76%. Regenerated plantlets were successfully transplanted in soil, with a success rate of 67%.
对松叶菊无菌苗的子叶切段在含有不同植物激素的不同培养基上进行培养。结果发现，在添加了10.0 mg/L萘乙酸（NAA）和0.2 mg/L 6-苄氨基腺嘌呤（6-BA）的MS基本培养基上能够高效诱导出愈伤组织。当将愈伤组织转移到含有2.0 mg/L 6-BA和0.2 mg/L NAA的MS培养基上时，可分化出芽。芽分化频率达到约85%。再生芽在添加了0.5 mg/L NAA的1/2MS培养基上生根。生根率约为76%。再生植株成功移栽到土壤中，成功率为67%。
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