Liu Li-sha, Zhang Xi-ling, Wang Lan, Zhang Yan-hong
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(10):797-800.
To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines.
Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones.
The MS medium with 0.5-1.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg x L(-1) IAA and 3 mg x L(-1) BA was suitable for the induction of calli. MS medium with 1.0 mg x L(-1) IAA and 2.0-3.0 mg x L(-1) BA was suitable for the subculture of calli. MS medium with 2.0 mg x L(-1) BA and 0.5 mg x L(-1) NAA was suitable for the differentiation of calli.
Aseptic seeding of G. stramines can be quickly propagated by shoot culture.
通过研究麻花艽组织培养技术，为大规模生产提供科学依据。
在添加不同激素的MS培养基上，从麻花艽无菌茎段诱导愈伤组织。
添加0.5 - 1.0 mg·L⁻¹ 苄氨基腺嘌呤（BA）和0.5 mg·L⁻¹ 萘乙酸（NAA）的MS培养基适合丛生芽的诱导和增殖。添加1.0 mg·L⁻¹ 吲哚乙酸（IAA）和3 mg·L⁻¹ BA的MS培养基适合愈伤组织的诱导。添加1.0 mg·L⁻¹ IAA和2.0 - 3.0 mg·L⁻¹ BA的MS培养基适合愈伤组织的继代培养。添加2.0 mg·L⁻¹ BA和0.5 mg·L⁻¹ NAA的MS培养基适合愈伤组织的分化。
麻花艽无菌苗可通过茎尖培养快速繁殖。
