Wu Li-Qin, Guo Shun-Xing, Xiao Pei-Gen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Zhongguo Zhong Yao Za Zhi. 2005 Jun;30(11):814-6.
To establish a protocol of rapid clonal propagation of Saussurea involucrate by using embryo as explants.
MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting.
All embryo explants started to grow adventitious buds within 45 days when they were cultured on the media supplemented with 0.02-0.1 mg x L(-1) NAA and 2 mg x L(-1) BA; The adventitious buds multiplicated within 30 days when they were transferred to the media containing 0.1-0.2 mg x L(-1) NAA and 2-3 mg x L(-1) BA; 92% of the adventitious buds rooted well after they were planted on the MS medium containing macroelements at half strength and 0.4 mg x L(-1) NAA for 25 days. The regenerated plantlets grew well after they were transplanted and the survival rate was up to 70%.
Plantlets of S. invducrate regenerated high frequence through adventitious bud.
以胚为外植体建立新疆雪莲快速克隆繁殖体系。
以MS培养基为基本培养基，添加BA和NAA，筛选不定芽诱导、不定芽增殖及生根的最佳激素组合。
在添加0.02 - 0.1 mg·L⁻¹ NAA和2 mg·L⁻¹ BA的培养基上培养，所有胚外植体45天内均开始生长不定芽；转接到含0.1 - 0.2 mg·L⁻¹ NAA和2 - 3 mg·L⁻¹ BA的培养基上，不定芽30天内增殖；将不定芽接种到含1/2大量元素和0.4 mg·L⁻¹ NAA的MS培养基上培养25天，92%的不定芽生根良好。再生植株移栽后生长良好，成活率达70%。
新疆雪莲通过不定芽途径高频再生植株。
