小眼畸形相关转录因子对小鼠肥大细胞蛋白酶-9的转录激活作用

Transcriptional activation of mouse mast cell protease-9 by microphthalmia-associated transcription factor.

作者信息

Murakami Masaru, Ikeda Teruo, Ogawa Kenji, Funaba Masayuki

机构信息

Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Sagamihara, Japan.

出版信息

Biochem Biophys Res Commun. 2003 Nov 7;311(1):4-10. doi: 10.1016/j.bbrc.2003.09.148.

DOI:10.1016/j.bbrc.2003.09.148

PMID:14575687

Abstract

We explored transcriptional regulation of mouse mast cell protease-9 (mMCP-9), which is implicated in inflammation of the jejunum during helminth infections and tissue remodeling of the uterus during pregnancy. Transcription was positively regulated by microphthalmia-associated transcription factor (MITF), a member of the basic helix-loop-helix-leucine zipper family that binds to the E-box, a CANNTG sequence. The most significant segment for positive regulation by MITF was nt -183 to -177 of the mMCP-9 promoter, CATCATG, which bound MITF-M. In addition, not only other MITF isoforms but also TFE3, another member of the family, activated mMCP-9 transcription through this nucleotide sequence inserted one base within the E-box.

摘要

我们研究了小鼠肥大细胞蛋白酶9（mMCP-9）的转录调控，该酶与蠕虫感染期间空肠的炎症以及妊娠期间子宫的组织重塑有关。转录由小眼相关转录因子（MITF）正向调控，MITF是碱性螺旋-环-螺旋-亮氨酸拉链家族的成员，可与E-box（CANNTG序列）结合。MITF正向调控的最显著区域是mMCP-9启动子的nt -183至-177，即CATCATG，它与MITF-M结合。此外，不仅其他MITF异构体，而且该家族的另一个成员TFE3，也通过插入E-box内一个碱基的该核苷酸序列激活mMCP-9转录。