Koubek K, Babusíková O
Institute of Haematology and Blood Transfusion, Clinical Department, U Nemocnice 1, 128 20 Prague 2, Czech Republic.
Sb Lek. 2003;104(2):183-97.
The expression of 82 cell surface molecules was analysed on pathological cells from 52 patients with acute lymphoblastic leukemia (T and B), acute myeloid leukemia (FAB: M0, M1, M2, M3, M4, M5), chronic myeloid leukemia, chronic myelo-monocytic leukemia) and non Hodgkin's lymphoma (T and B types). We have selected patients with a high leukocyte count and percentage of blasts. Staining for membrane molecules was done by the immunofluorescence method and evaluated by flow cytometry. The findings indicate that expression of membrane molecules on pathological cells is quantitatively and qualitatively different in individual cases. The leukemia/lymphoma cells in their crude from represent the main phenotypes of normal haematopoietic cells, which reveal the great diversity of immunophenotypes within the main functional characterization of blood malignancies. The immunophenotype heterogeneity of leukemic cells has proved to be much greater than the match with existing classification criteria, this fact could raise the necessity for further functional evaluation and specification of immunophenotyping of the leukemia/lymphoma cells. The concept of explanation of pathogenesis and pathophysiology of different types of leukemia/lymphoma cells on the basis that they are derived from normal haematopoiesis must be accepted, because the number of membrane markers and their functional properties are correspondingly convincing.
对52例急性淋巴细胞白血病（T型和B型）、急性髓系白血病（FAB：M0、M1、M2、M3、M4、M5）、慢性髓系白血病、慢性粒单核细胞白血病患者以及非霍奇金淋巴瘤（T型和B型）患者的病理细胞进行了82种细胞表面分子的表达分析。我们选择了白细胞计数和原始细胞百分比高的患者。通过免疫荧光法对膜分子进行染色，并通过流式细胞术进行评估。研究结果表明，病理细胞上膜分子的表达在个体病例中在数量和质量上存在差异。白血病/淋巴瘤细胞的原始形态代表了正常造血细胞的主要表型，这揭示了血液恶性肿瘤主要功能特征内免疫表型的巨大多样性。白血病细胞的免疫表型异质性已被证明远大于与现有分类标准的匹配度，这一事实可能增加对白血病/淋巴瘤细胞免疫表型进行进一步功能评估和细化的必要性。基于不同类型白血病/淋巴瘤细胞来源于正常造血这一观点来解释其发病机制和病理生理学的概念必须被接受，因为膜标志物的数量及其功能特性具有相应的说服力。
