基因毒性应激诱导的人类Alu元件逆转座

Human Alu element retrotransposition induced by genotoxic stress.

作者信息

Hagan Christy R, Sheffield Rebecca F, Rudin Charles M

机构信息

Biological Sciences Division and Committee on Cancer Biology, University of Chicago, Chicago, Illinois 60637, USA.

出版信息

Nat Genet. 2003 Nov;35(3):219-20. doi: 10.1038/ng1259. Epub 2003 Oct 26.

DOI:10.1038/ng1259

PMID:14578886

Abstract

Alu elements are found exclusively in primate species and comprise over 10% of the human genome. To better define the mechanisms responsible for Alu replication, we introduced a human Alu element into mouse cells. We report that Alu retrotransposition can be induced in mouse cells by exposure to the topoisomerase II inhibitor etoposide and is mediated in trans by endogenous mouse long interspersed elements (LINEs).

摘要

Alu元件仅在灵长类物种中发现，占人类基因组的10%以上。为了更好地确定Alu复制的机制，我们将一个人类Alu元件导入小鼠细胞。我们报告，通过暴露于拓扑异构酶II抑制剂依托泊苷，Alu逆转录转座可在小鼠细胞中被诱导，并且由内源性小鼠长散在元件（LINEs）介导进行反式作用。