大鼠髓鞘碱性蛋白17-kDa同工型编码cDNA的鉴定与表征

Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein.

作者信息

Matheus Luisa, Blair G Eric

机构信息

School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.

出版信息

Biochim Biophys Acta. 2003 Oct 20;1630(1):47-53. doi: 10.1016/j.bbaexp.2003.08.010.

DOI:10.1016/j.bbaexp.2003.08.010

PMID:14580679

Abstract

The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

摘要

髓鞘碱性蛋白（MBP）是髓鞘膜的主要蛋白质。多种MBP mRNA和蛋白质异构体通过可变RNA剪接产生。在此，我们描述了从大鼠（褐家鼠）中分离和鉴定编码17 kDa MBP异构体的cDNA克隆。该异构体是一种由MBP基因的外显子1、3、4、6和7组成的158个氨基酸的蛋白质。对脑mRNA的RT-PCR分析表明，编码17 kDa异构体的转录本在出生后发育早期，即产后7天内表达水平较高。