Takahashi N, Roach A, Teplow D B, Prusiner S B, Hood L
Cell. 1985 Aug;42(1):139-48. doi: 10.1016/s0092-8674(85)80109-4.
A mouse cosmid library was screened with a cDNA clone for rat small (M, 14,000) myelin basic protein (MBP). The entire MBP gene was isolated. Five exons were detected with the cDNA clone, which encode the Mr 14,000 MBP. A sixth exon, detected using a synthetic oligonucleotide, encodes the 41 amino acids specific to the Mr 18,500 form of MBP. Splicing together all six exons would give rise to Mr 18,500 MBP, while an mRNA omitting exon V would be translated to produce Mr 14,000 MBP. Further experiments indicate that there is probably a single MBP gene in the mouse genome, and that there is a single major 5' end for mouse MBP transcripts, 47 bp 5' of the initiator methionine codon.
用大鼠小分子量（14000）髓鞘碱性蛋白（MBP）的cDNA克隆筛选小鼠黏粒文库。分离出了完整的MBP基因。用该cDNA克隆检测到5个外显子，它们编码14000分子量的MBP。用合成寡核苷酸检测到的第6个外显子，编码18500分子量形式MBP特有的41个氨基酸。将所有6个外显子拼接在一起会产生18500分子量的MBP，而缺失外显子V的mRNA将被翻译产生14000分子量的MBP。进一步的实验表明，小鼠基因组中可能只有一个MBP基因，并且小鼠MBP转录本有一个单一的主要5′端，位于起始甲硫氨酸密码子的5′端47 bp处。
