Eaton G J, Custer R P, Johnson F N, Stabenow K T
Am J Pathol. 1978 Jan;90(1):173-86.
Mice of five inbred strains (BALB/c, C3H, C3Hf, DBA/2, and C57BL/6) of both sexes, mated and virginal, were examined for the incidence, severity, and location of dystrophic cardiac calcinosis (DCC) at various ages. Three hybrids, B6C3F1, C3B6F1, and CC3F1 of both sexes, all mated, were likewise studied. Excepting DBA/2, females of the inbred strains acquired the lesion at a much earlier age than males; DCC appeared in young DBA/s mice of both sexes. DCC in BALB/c mice was almost exclusively epicardial and occurred with equal frequency and severity in mated males and females, with higher incidence but lesser extent in virginal females. The occurrence was highest, the degree most severe, and the location exclusively myocardial in C3H and C3Hf mated females, irrespective of parity, whereas virginal females of these strains were entirely free of disease even after administration of exogenous progesterone. Involvement of males, also myocardial, was relatively minimal, especially in C3Hf mice. Over half the DBA/2 mice were affected, regardless of sex or mating; calcinosis appeared in the epicardium and/or myocardium, predominantly in the myocardium. Strain C57BL/6 was completely devoid of the lesion, as were the two hybrids thereof, B6C3F1 and C3B6F1. The hybrid of BALB/c and C3H showed a high incidence of minimal involvement, exclusively myocardial and limited to breeding females, indicating dominance of the C3H gene(s). Renal calcinosis was uncommon among BALB/c mice but was frequently found in C3H, C3Hf, and DBA/2 strains. Pulmonary calcinosis was rare and limited to C3H and C3Hf female breeders. Mated C3H females fed increasing amounts of fat showed a concomitant rise in incidence and severity of the cardiac lesions. Progression of the lesion from necrotic myocardial fibers to fibrocalcific masses is illustrated, as is formation of the renal deposits.
对五个近交系（BALB/c、C3H、C3Hf、DBA/2和C57BL/6）的雄性和雌性小鼠进行了检查，这些小鼠包括交配过的和未交配的处女鼠，以研究不同年龄阶段营养不良性心脏钙化（DCC）的发生率、严重程度和位置。同样对三个杂交系的雄性和雌性小鼠（B6C3F1、C3B6F1和CC3F1，均为交配过的）进行了研究。除DBA/2外，近交系雌性小鼠比雄性小鼠更早出现病变；DCC出现在年轻的DBA/2雌雄小鼠中。BALB/c小鼠的DCC几乎只出现在心外膜，交配的雄性和雌性小鼠中出现的频率和严重程度相同，处女雌性小鼠的发生率较高但程度较轻。C3H和C3Hf交配的雌性小鼠中DCC的发生率最高、程度最严重且只出现在心肌，与产次无关，而这些品系的处女雌性小鼠即使在给予外源性孕酮后也完全没有疾病。雄性小鼠的受累情况（同样是心肌）相对较少，尤其是在C3Hf小鼠中。超过一半的DBA/2小鼠受到影响，无论性别或是否交配；钙化出现在心外膜和/或心肌，主要在心肌。C57BL/6品系以及其两个杂交系B6C3F1和C3B6F1完全没有这种病变。BALB/c和C3H的杂交系显示出轻度受累的高发生率，只出现在心肌且仅限于繁殖雌性小鼠，表明C3H基因占主导地位。肾钙化在BALB/c小鼠中不常见，但在C3H、C3Hf和DBA/2品系中经常发现。肺钙化很少见，仅限于C3H和C3Hf雌性繁殖小鼠。喂食脂肪量增加的交配C3H雌性小鼠心脏病变的发生率和严重程度随之升高。展示了病变从坏死心肌纤维发展到纤维钙化团块的过程，以及肾沉积物的形成过程。
