Lim Kye-Taek, Lee Sei-Jung, Heo Kyung-Sun, Lim Kwang
#521, Molecular Biochemistry Laboratory & Biodefensive Substances Group, Institute of Biotechnology, Chonnam National University, 300 Yongbong-Dong, Bukgu, Kwangju 500757, South Korea.
Toxicol Lett. 2003 Dec 10;145(3):261-71. doi: 10.1016/s0378-4274(03)00304-7.
Glycoprotein of Rhus verniciflua Stokes (RVS glycoprotein) was isolated and identified using SDS-PAGE. To study the anti-apoptotic effects of RVS glycoprotein on mouse splenocytes, splenocytes were exposed to 100 nM TPA (61.68 ng/ml) for 3 h with or without RVS glycoprotein (100 microg/ml). Results from our experiment showed that RVS glycoprotein protects from splenocyte apoptosis induced by 12-O-tetradecanoylphorbol 13-acetate (TPA). We also studied the effects of RVS glycoprotein on the proliferation of T/B cells and the production of cytokines. Our results showed that Concanavalin A (Con A)-induced T cell proliferation and the production of interleukin-2 (IL-2)/interleukin-4 (IL-4) were reduced, and that Lipopolysaccharide (LPS)-induced B cell proliferation and the Tumor necrosis factor alpha (TNF-alpha) were reduced significantly by the addition of 50 microg/ml RVS glycoprotein (P<0.01), compared to the control. These results indicate that RVS glycoprotein has the capacity to modulate apoptosis, cytokine production and T/B cell proliferation in splenocytes.
采用SDS - PAGE法分离并鉴定了漆树糖蛋白（RVS糖蛋白）。为研究RVS糖蛋白对小鼠脾细胞的抗凋亡作用，将脾细胞暴露于100 nM TPA（61.68 ng/ml）中3小时，同时加入或不加入RVS糖蛋白（100 μg/ml）。我们的实验结果表明，RVS糖蛋白可保护脾细胞免受12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）诱导的凋亡。我们还研究了RVS糖蛋白对T/B细胞增殖及细胞因子产生的影响。我们的结果显示，与对照组相比，加入50 μg/ml RVS糖蛋白后，伴刀豆球蛋白A（Con A）诱导的T细胞增殖以及白细胞介素 - 2（IL - 2）/白细胞介素 - 4（IL - 4）的产生减少，脂多糖（LPS）诱导的B细胞增殖以及肿瘤坏死因子α（TNF - α）显著减少（P<0.01）。这些结果表明，RVS糖蛋白具有调节脾细胞凋亡、细胞因子产生及T/B细胞增殖的能力。
