Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University, Seoul, Republic of Korea.
Phytother Res. 2010 Feb;24(2):288-94. doi: 10.1002/ptr.2941.
Rhus verniciflua Stokes (RVS) is a traditional medicine used in Korea, Japan and China to treat various diseases including catharsis, diaphoretic gastritis and stomach cancer. However, the effects of RVS on allergic inflammatory diseases are unknown to date. This study showed the antiallergic inflammatory effects of RVS on human mast cells (HMC-1) which were stimulated by phorbol myristate acetate (PMA) and calcium ionophore A23187. RVS inhibited the expressions of TNF-alpha, IL-6 and IL-8 that were stimulated by treatment with both PMA and A23187. Among the mitogen-activated protein kinases (MAPKs), extracts of RVS suppressed the phosphorylation of ERK and p38, whereas RVS increased the phosphorylation of JNK in HMC-1. Consistent with the regulation of MAPKs, it was found that RVS inhibited the nuclear translocation of nuclear factor (NF)-kappaB via inhibition of the phosphorylation of IkappaB-alpha, which are important processes in controlling inflammatory responses. Taken together, these results suggest that RVS modulates the expressions of signal molecules related to allergic inflammatory responses mainly through the ERK signaling pathway, suggesting that RVS could be used as a treatment for mast cell-derived allergic inflammatory diseases.
漆树（Rhus verniciflua Stokes，RVS）是一种传统药物，在韩国、日本和中国被用于治疗各种疾病，包括泻下、解表性胃炎和胃癌。然而，目前还不清楚 RVS 对过敏性炎症性疾病的影响。本研究显示，RVS 对人肥大细胞（HMC-1）具有抗过敏性炎症作用，这些肥大细胞是由佛波醇肉豆蔻酸酯（PMA）和钙离子载体 A23187 刺激产生的。RVS 抑制了 PMA 和 A23187 联合刺激产生的 TNF-α、IL-6 和 IL-8 的表达。在丝裂原活化蛋白激酶（MAPKs）中，RVS 提取物抑制了 ERK 和 p38 的磷酸化，而 RVS 增加了 HMC-1 中 JNK 的磷酸化。与 MAPKs 的调节一致，研究发现 RVS 通过抑制 IkappaB-α的磷酸化来抑制核转录因子（NF）-kappaB 的核易位，这是控制炎症反应的重要过程。综上所述，这些结果表明，RVS 通过 ERK 信号通路调节与过敏性炎症反应相关的信号分子的表达，提示 RVS 可用于治疗肥大细胞来源的过敏性炎症性疾病。
