Frändberg Emma, Pitt John, Olsen Monica
Microbiology Division, National Food Administration, Box 622, SE-751 26 Uppsala, Sweden.
Int J Food Microbiol. 2003 Dec 15;89(1):99-102. doi: 10.1016/s0168-1605(03)00293-9.
AFPA culture medium, which is used for recognition of Aspergillus flavus and A. parasiticus, has been validated in a collaborative study including nine laboratories located in Australia, Brazil, Denmark, The Netherlands, Sweden and United Kingdom. Three freeze-dried fungal mixtures, containing A. flavus/A. parasiticus and background fungi, were produced and checked for homogeneity. The coefficients of variance were low, ranging from 0.81% to 1.09% for total fungal counts and between 2.50% and 2.72% for counts of A. flavus/A. parasiticus. The laboratories analysed the contents of two vials of each mixture on commercial A. flavus and A. parasiticus agar (AFPA), in-house-made AFPA, and on a standard media, dichloran 18% glycerol agar (DG18). Reproducibility values for counts of A. flavus/A. parasiticus indicated no differences between the commercial AFPA and the in-house-made AFPA. Variation between laboratories was low, indicating that the medium was effective in use. Reproducibility values for DG18 were higher. There were no differences in counts of A. flavus/A. parasiticus on AFPA and DG18. However, DG18 gave slightly higher total fungal counts compared to AFPA.
用于鉴定黄曲霉和寄生曲霉的AFPA培养基，已在一项合作研究中得到验证，该研究涉及位于澳大利亚、巴西、丹麦、荷兰、瑞典和英国的九个实验室。制备了三种含有黄曲霉/寄生曲霉和背景真菌的冻干真菌混合物，并检查了其均匀性。总真菌计数的变异系数较低，范围为0.81%至1.09%，黄曲霉/寄生曲霉计数的变异系数在2.50%至2.72%之间。各实验室在商用黄曲霉和寄生曲霉琼脂（AFPA）、自制AFPA以及标准培养基二氯苯18%甘油琼脂（DG18）上分析了每种混合物的两个小瓶的内容物。黄曲霉/寄生曲霉计数的重现性值表明，商用AFPA和自制AFPA之间没有差异。实验室间的差异较小，表明该培养基使用效果良好。DG18的重现性值较高。AFPA和DG18上黄曲霉/寄生曲霉的计数没有差异。然而，与AFPA相比，DG18的总真菌计数略高。
