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用于花生、玉米粉和豇豆中总酵母和霉菌以及潜在产黄曲霉毒素曲霉菌计数的曲霉菌鉴别培养基与黄曲霉/寄生曲霉琼脂的比较

Comparison of Aspergillus Differential Medium and Aspergillus flavus / parasiticus Agar for Enumerating Total Yeasts and Molds and Potentially Aflatoxigenic Aspergilli in Peanuts, Corn Meal and Cowpeas.

作者信息

Beuchat L R

机构信息

Department of Food Science, University of Georgia Agricultural Experiment Station, Experiment, Georgia 30212.

出版信息

J Food Prot. 1984 Jul;47(7):512-519. doi: 10.4315/0362-028X-47.7.512.

DOI:10.4315/0362-028X-47.7.512
PMID:30934468
Abstract

Aspergillus differential medium (ADM) and Aspergillus flavus / parasiticus agar (AFPA) were compared for enumerating total yeasts and molds as well as the A. flavus group in raw peanuts, corn meal and cowpeas. Colony counts were made after plates were incubated at 30 and 37°C; incubation times of 42-44 and 70-72 h were evaluated. Incubation at 30°C enhanced recovery of yeasts and molds compared to incubation at 37°C. Incubation for 42-44 h at 37°C on ADM or AFPA was optimum for detecting A. flavus in corn meal. Use of AFPA for detecting A. flavus / parasiticus may have some advantage over ADM, since orange-yellow color development of reverse colonies is quicker and more intense on AFPA.

摘要

对曲霉鉴别培养基(ADM)和黄曲霉/寄生曲霉琼脂(AFPA)进行了比较,以计数生花生、玉米粉和豇豆中的总酵母和霉菌以及黄曲霉组。平板在30℃和37℃培养后进行菌落计数;评估了42 - 44小时和70 - 72小时的培养时间。与37℃培养相比,30℃培养可提高酵母和霉菌的回收率。在ADM或AFPA上于37℃培养42 - 44小时是检测玉米粉中黄曲霉的最佳条件。使用AFPA检测黄曲霉/寄生曲霉可能比ADM有一些优势,因为AFPA上反向菌落的橙黄色显色更快且更强烈。

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