Xu Li Xiao, Shen Yun Xiu, Wang Huai You, Jiang Ji Gang, Xiao Yan
Department of Chemistry, Shandong Normal University, Jinan 250014, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2003 Nov;59(13):3103-10. doi: 10.1016/s1386-1425(03)00125-2.
Spectrophotometric determination of procaine hydrochloride is described. The procaine hydrochloride reacts with 1,2-naphthoquinone-4-sulfonic acid in pH 3.60 buffer solution to form a salmon pink compound, and its maximum absorption wavelength is at 484 nm, epsilon(484)=5.22 x 10(3).The absorbance for procaine hydrochloride from 0.30 to 100 microg ml(-1) obeys Beer's law. The linear regression equation of the calibration graph is C=19.23A-0.03, with a linear regression correlative coefficient is 0.9996, the detection limit is 0.28 microg ml(-1); recovery is from 98.0 to 105.2%. Effects of pH, surfactant, organic solvent, foreign ions, and standing time on the determination of procaine hydrochloride have been examined. This method is rapid and simple, and can be used for the determination of procaine hydrochloride in injection solution of procaine hydrochloride. The results obtained by this method agreed with those by the official method (dead-stop titration).
描述了盐酸普鲁卡因的分光光度测定法。盐酸普鲁卡因在pH 3.60的缓冲溶液中与1,2-萘醌-4-磺酸反应形成鲑鱼粉红色化合物，其最大吸收波长为484 nm，ε(484)=5.22×10³。盐酸普鲁卡因在0.30至100 μg ml⁻¹范围内的吸光度符合比尔定律。校准曲线的线性回归方程为C = 19.23A - 0.03，线性回归相关系数为0.9996，检测限为0.28 μg ml⁻¹；回收率为98.0%至105.2%。考察了pH、表面活性剂、有机溶剂、外来离子和静置时间对盐酸普鲁卡因测定的影响。该方法快速简便，可用于盐酸普鲁卡因注射液中盐酸普鲁卡因的测定。该方法所得结果与法定方法（永停滴定法）一致。
