Li Quanmin, Zhang Huanhuan
College of Chemical and Environmental Sciences, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan 453007, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jul;70(2):284-9. doi: 10.1016/j.saa.2007.07.056. Epub 2007 Aug 14.
A rapid, simple and sensitive method for the determination of aminophylline (Ami) using sodium 1, 2-naphthoquine-4-sulfonate (NQS) and methanol is established in this paper. It is based on the fact that a russety product can be formed by the reaction between aminophylline (Ami) and sodium 1, 2-naphthoquine-4-sulfonate (NQS) in pH 13.00 buffer solution. When methanol is added to the solution, the sensitivity of the color development reaction between Ami and NQS is improved, and the color of the system of NQS-Ami becomes a salmon pink. Beer's law is obeyed in a range of 4.97-69.5 microg ml(-1) of Ami at the maximum absorption of 453 nm (epsilon=4.87 x 10(3) l mol(-1) cm(-1)). The linear regression equation of the calibration curve is A=0.14458+0.00832C (microg ml(-1)), with a linear regression correlation coefficient of 0.9944. The detection limit is 0.7 microg ml(-1) (3sigma/k), R.S.D. is 1.1% and the recovery rate is in range of 92.5-105%. Furthermore, this method has been successfully applied to the determination of Ami in pharmaceutical samples.
本文建立了一种快速、简便、灵敏的用1,2-萘醌-4-磺酸钠（NQS）和甲醇测定氨茶碱（Ami）的方法。该方法基于氨茶碱（Ami）与1,2-萘醌-4-磺酸钠（NQS）在pH 13.00缓冲溶液中反应可形成棕红色产物这一事实。当向溶液中加入甲醇时，Ami与NQS显色反应的灵敏度提高，NQS-Ami体系的颜色变为鲑鱼粉红色。在453 nm最大吸收波长处，Ami浓度在4.97 - 69.5 μg ml⁻¹范围内符合比尔定律（ε = 4.87×10³ l mol⁻¹ cm⁻¹）。校准曲线的线性回归方程为A = 0.14458 + 0.00832C（μg ml⁻¹），线性回归相关系数为0.9944。检测限为0.7 μg ml⁻¹（3σ/k），相对标准偏差为1.1%，回收率在92.5% - 105%范围内。此外，该方法已成功应用于药物样品中Ami的测定。
