Ghanem K M, Yusef H H
Botany Department, Faculty of Science, Alexandria University, Egypt.
Can J Microbiol. 1992 Aug;38(8):753-7. doi: 10.1139/m92-122.
Reichstein's compound S was successfully converted to prednisolone in a single-step fermentation using a mixed culture of Curvularia lunata and Mycobacterium smegmatis. Introducing additional medium at the time of bacterial inoculation and increasing the M. smegmatis inoculum to 8% were necessary for maximal dehydrogenation of cortisol to prednisolone (86%). However, beef extract, corn-steep solids, and malt extract were inhibitory to the dehydrogenase activity and stimulatory to hydroxylase. Of the vitamins tested, nicotinic acid and riboflavin at 0.2 and 1.13 mg/L, respectively, resulted in maximum transformation of Reichstein's compound S (100%) and optimized prednisolone yields (92%) in the mixed culture. The trace elements present in the medium were sufficient for maximal transformation, and there was no need for an exogenous supply. Addition of ATP, sodium acetate, and NAD inhibited the dehydrogenation reaction.
利用新月弯孢霉和耻垢分枝杆菌的混合培养物，在一步发酵中成功地将 Reichstein 化合物 S 转化为泼尼松龙。在细菌接种时添加额外的培养基，并将耻垢分枝杆菌接种量增加到 8%，对于将皮质醇最大程度地脱氢为泼尼松龙（86%）是必要的。然而，牛肉浸出物、玉米浆固体和麦芽提取物对脱氢酶活性有抑制作用，对羟化酶有刺激作用。在所测试的维生素中，烟酸和核黄素分别以 0.2 和 1.13 mg/L 的浓度添加时，混合培养物中 Reichstein 化合物 S 的转化达到最大值（100%），泼尼松龙产量得到优化（92%）。培养基中存在的微量元素足以实现最大程度的转化，无需外源供应。添加 ATP、醋酸钠和 NAD 会抑制脱氢反应。
