Huang S H, Xu S W, Fa Y H
Wei Sheng Wu Xue Bao. 1989 Feb;29(1):68-71.
The conversion of 16 alpha-methyl-Reichstein's compound S 21-acetate (I) to 16 alpha-methyl-hydrocortisone (II) by the mycelium Curvularia lunata AS3.4381 was studied. Maximal 11 beta-hydroxylating activity of the mycelium was found during cultivation of the microorganism by 24h. Inhibition of the ethanol to the 11 beta-hydroxylating activity was obvious. The yield of 55.4% (II) (W/W) could be achieved during conversion of 0.15% substrate at 72 h.
研究了新月弯孢霉AS3.4381菌丝体将16α-甲基- Reichstein化合物S 21-乙酸酯（I）转化为16α-甲基氢化可的松（II）的过程。在微生物培养24小时期间，发现菌丝体具有最大的11β-羟化活性。乙醇对11β-羟化活性的抑制作用明显。在72小时内转化0.15%的底物时，可实现55.4%（II）（重量/重量）的产率。
