采用石墨炉塞曼原子吸收光谱法直接测定血清中的锌。
Direct determination of zinc in serum by Zeeman atomic absorption spectrometry with a graphite furnace.
作者信息
D'Haese P C, Lamberts L V, Vanheule A O, De Broe M E
机构信息
Department of Nephrology-Hypertension, University Hospital Antwerp, University of Antwerp, Edegem, Belgium.
出版信息
Clin Chem. 1992 Dec;38(12):2439-43.
We developed a precise and accurate graphite furnace atomic absorption spectrometric method for the direct determination of zinc in serum. Serum samples are analyzed after 20-fold dilution with water of ultrapure analytical grade. No other reagent is used, from the moment of sampling until measurement. During atomization, the argon flow is kept at 150 mL/min instead of gas stop, to decrease the sensitivity and thus allow lower dilution ratios. Zinc concentrations are determined against a serum-matched calibration curve. Graphite tubes are uncoated and no L'vov platform is used. Between-run CVs were 5.9%, 3.5%, and 1.9% for serum zinc concentrations of 0.93, 1.15, and 1.43 mg/L, respectively. The characteristic mass was 9 pg, and the detection limit (meanblank + 3SDblank) was 0.060 mg/L.
我们开发了一种精确且准确的石墨炉原子吸收光谱法，用于直接测定血清中的锌。血清样品用超纯分析级水进行20倍稀释后进行分析。从采样到测量期间，不使用其他试剂。在原子化过程中，氩气流速保持在150 mL/min而不是停止通气，以降低灵敏度，从而允许更低的稀释倍数。锌浓度通过与血清匹配的校准曲线来测定。石墨管未涂层，且未使用热解涂层石墨管平台。对于血清锌浓度分别为0.93、1.15和1.43 mg/L的情况，批间变异系数分别为5.9%、3.5%和1.9%。特征质量为9 pg，检测限（平均空白值 + 3倍空白值标准差）为0.060 mg/L。
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