Schmiedeke T, Stoeckl F, Muller S, Sugisaki Y, Batsford S, Woitas R, Vogt A
Institute of Medical Microbiology, Freiburg, Germany.
Clin Exp Immunol. 1992 Dec;90(3):453-8. doi: 10.1111/j.1365-2249.1992.tb05867.x.
Two types of lupus mice, NZB/NZW F1 female hybrids and mice with graft-versus-host disease (GVHD), were studied. Histones H3 and H2A were detected by immunofluorescence in glomeruli of 22/22 proteinuric GVHD and 8/12 proteinuric NZB/W F1 female mice; in non-proteinuric animals, 3/5 GVHD and 2/27 NZB/W F1 female were positive. Using antibodies to histone peptides it was shown that mainly the N-terminal regions of histones H3 and H2A were exposed in glomerular deposits. Western blot analysis revealed antibodies to histone subfractions in sera of 33/34 lupus mice that developed proteinuria. This study provides evidence that histones are involved in the pathogenesis of lupus nephritis.
研究了两种狼疮小鼠，即NZB/NZW F1雌性杂交小鼠和患有移植物抗宿主病（GVHD）的小鼠。通过免疫荧光在22/22出现蛋白尿的GVHD小鼠和8/12出现蛋白尿的NZB/W F1雌性小鼠的肾小球中检测到组蛋白H3和H2A；在无蛋白尿的动物中，3/5的GVHD小鼠和2/27的NZB/W F1雌性小鼠呈阳性。使用针对组蛋白肽的抗体表明，主要是组蛋白H3和H2A的N端区域暴露于肾小球沉积物中。蛋白质印迹分析显示，在33/34出现蛋白尿的狼疮小鼠血清中存在针对组蛋白亚组分的抗体。这项研究提供了证据，证明组蛋白参与狼疮性肾炎的发病机制。
