Muller S, Briand J P, Van Regenmortel M H
Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, Strasbourg, France.
Proc Natl Acad Sci U S A. 1988 Nov;85(21):8176-80. doi: 10.1073/pnas.85.21.8176.
Sera from patients with systemic lupus erythematosus (SLE) were shown to react with both ubiquitin and a synthetic fragment of it (residues 22-45) in an ELISA and with ubiquitin in immunoblotting experiments. Close to 80% of lupus patients possessed ubiquitin antibodies, whereas only 55% of them possessed native DNA antibodies, a marker of SLE. Less than 16% of patients with other rheumatic autoimmune diseases possessed antibodies to ubiquitin. Our results indicate that the combined measurement of antibodies to native DNA and to ubiquitin could appreciably increase the detection of SLE cases (up to 85% in our study). It is suggested that ubiquitin, a heat shock protein, could be involved in antibody formation against ubiquitin-protein conjugates present during cellular injury and that this represents a major characteristic of the autoimmune response in SLE.
在酶联免疫吸附测定（ELISA）中，系统性红斑狼疮（SLE）患者的血清显示出与泛素及其一个合成片段（第22 - 45位氨基酸残基）发生反应，并且在免疫印迹实验中与泛素发生反应。近80%的狼疮患者拥有泛素抗体，而其中只有55%拥有天然DNA抗体（SLE的一个标志物）。其他风湿性自身免疫疾病患者中，不到16%拥有抗泛素抗体。我们的结果表明，联合检测天然DNA抗体和泛素抗体能够显著提高SLE病例的检出率（在我们的研究中高达85%）。有人提出，作为一种热休克蛋白的泛素，可能参与针对细胞损伤时出现的泛素 - 蛋白质缀合物的抗体形成，并且这代表了SLE自身免疫反应的一个主要特征。
