[塔玛亚历山大藻的氮磷吸收与生长特性]

[Nitrogen and phosphorus absorption and growth characteristics of Alexandrium tamarense].

作者信息

Shi Yanjun, Hu Hanhua, Ma Runyu, Cong Wei, Cai Zhaoling

机构信息

School of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2003 Jul;14(7):1143-6.

PMID:14587339

Abstract

The uptake of nitrate and phosphate by Alexandrium tamarense and its growth characteristics were studied in laboratory cultures, which were conducted using environmentally realistic levels of physical factors during red tide occurrences. The results indicated that the specific growth rate had little difference under three nitrate levels (low-nitrate, 0.0882 mmol.L-1; middle-nitrate, 0.882 mmol.L-1; high-nitrate, 2.646 mmol.L-1). The cell yield was the lowest at low-nitrate, a maximum yield was achieved at the middle-nitrate, which increased by 44.7% and 53.6% respectively, compared with high-nitrate and low-nitrate. The cell yield increased with increasing phosphate concentrations in cultures (low-phosphate, 0.0036 mmol.L-1; middle-phosphate, 0.036 mmol.L-1; high-phosphate, 0.108 mmol.L-1), and the maximum yield (17200 cell.ml-1) was observed at high-phosphate concentration, while the maximum specific growth rate occurred at middle-phosphate. The growth conditions had a significant effect on the uptake rate of nitrate and phosphate by cells, and the cells grown in low-nitrate and low-phosphate cultures had a higher uptake rate. Further experiment suggested that lower ratio of N/P promoted the propagation of cells, and the supplementation of nitrate at later exponential growth phase had a positive effect to the accumulation of biomass.

摘要

在实验室培养条件下，模拟赤潮发生时的环境物理因子水平，研究了塔玛亚历山大藻对硝酸盐和磷酸盐的吸收及其生长特性。结果表明，在三种硝酸盐水平（低硝酸盐，0.0882 mmol·L⁻¹；中硝酸盐，0.882 mmol·L⁻¹；高硝酸盐，2.646 mmol·L⁻¹）下，比生长速率差异不大。细胞产量在低硝酸盐水平下最低，在中硝酸盐水平下达到最高，与高硝酸盐和低硝酸盐相比，分别增加了44.7%和53.6%。细胞产量随着培养物中磷酸盐浓度的增加而增加（低磷酸盐，0.0036 mmol·L⁻¹；中磷酸盐，0.036 mmol·L⁻¹；高磷酸盐，0.108 mmol·L⁻¹），在高磷酸盐浓度下观察到最大产量（17200个细胞·ml⁻¹），而最大比生长速率出现在中磷酸盐水平。生长条件对细胞对硝酸盐和磷酸盐的吸收速率有显著影响，在低硝酸盐和低磷酸盐培养条件下生长的细胞具有较高的吸收速率。进一步的实验表明，较低的N/P比促进了细胞的繁殖，在指数生长后期补充硝酸盐对生物量的积累有积极作用。