Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites.
作者信息
Webb Michael E, Stephens Elaine, Smith Alison G, Abell Chris
机构信息
University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
出版信息
Chem Commun (Camb). 2003 Oct 7(19):2416-7. doi: 10.1039/b308182f.
DOI:10.1039/b308182f
PMID:14587709
Abstract
The binding affinity of aspartate decarboxylase has been probed using MALDI-TOF spectrometry; adducts formed covalently in the active site were detected by MALDI-TOF mass spectrometry after incubation of the enzyme with a range of potential ligands in the presence of NaCNBH3; this has highighted key structural features which will aid design of potential inhibitors.
摘要
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