大肠杆菌天冬氨酸α-脱羧酶Asn72Ala的结构：探究Asn72在丙酮酸辅因子形成中的作用
Structure of Escherichia coli aspartate α-decarboxylase Asn72Ala: probing the role of Asn72 in pyruvoyl cofactor formation.
作者信息
Webb Michael E, Lobley Carina M C, Soliman Fatima, Kilkenny Mairi L, Smith Alison G, Blundell Tom L, Abell Chris
机构信息
University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Apr 1;68(Pt 4):414-7. doi: 10.1107/S1744309112009487. Epub 2012 Mar 28.
Abstract
The crystal structure of the Asn72Ala site-directed mutant of Escherichia coli aspartate α-decarboxylase (ADC) has been determined at 1.7 Å resolution. The refined structure is consistent with the presence of a hydrolysis product serine in the active site in place of the pyruvoyl group required for catalysis, which suggests that the role of Asn72 is to protect the ester formed during ADC activation from hydrolysis. In previously determined structures of activated ADC, including the wild type and other site-directed mutants, the C-terminal region of the protein is disordered, but in the Asn72Ala mutant these residues are ordered owing to an interaction with the active site of the neighbouring symmetry-related multimer.
摘要
已通过1.7 Å分辨率确定了大肠杆菌天冬氨酸α-脱羧酶（ADC）的Asn72Ala定点突变体的晶体结构。优化后的结构与活性位点中存在水解产物丝氨酸一致，该丝氨酸取代了催化所需的丙酮酰基团，这表明Asn72的作用是保护ADC激活过程中形成的酯不被水解。在先前确定的包括野生型和其他定点突变体在内的活化ADC结构中，蛋白质的C端区域是无序的，但在Asn72Ala突变体中，由于与相邻对称相关多聚体的活性位点相互作用，这些残基是有序的。
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