Sacconi L, Froner E, Antolini R, Taghizadeh M R, Choudhury A, Pavone F S
University of Trento, Via Sommarive 14, 38050 Povo, Trento, Italy.
Opt Lett. 2003 Oct 15;28(20):1918-20. doi: 10.1364/ol.28.001918.
Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds.
多光子多焦点显微镜（MMM）通常是通过将振镜扫描仪与微透镜阵列、微透镜阵列旋转盘以及带有扫描镜异步光栅扫描的级联分束器相结合来实现的。在此，我们描述了一种简洁紧凑的MMM的实现方法，该方法利用了一种高衍射效率的衍射光学元件，它能生成具有均匀强度的多点网格，从而在以适当速度对活细胞成像时实现更高的保真度。
