多光子显微镜设计实用指南。
A pragmatic guide to multiphoton microscope design.
作者信息
Young Michael D, Field Jeffrey J, Sheetz Kraig E, Bartels Randy A, Squier Jeff
机构信息
Center for Microintegrated Optics for Advanced Biological Control, Department of Physics, Colorado School of Mines, 1500 Illinois Street, Golden, Colorado 80401, USA.
W. M. Keck Laboratory for Raman Imaging of Cell-to-Cell Communications, Colorado State University, Fort Collins, Colorado 80523, USA; Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
出版信息
Adv Opt Photonics. 2015 Jun 30;7(2):276-378. doi: 10.1364/AOP.7.000276.
Multiphoton microscopy has emerged as a ubiquitous tool for studying microscopic structure and function across a broad range of disciplines. As such, the intent of this paper is to present a comprehensive resource for the construction and performance evaluation of a multiphoton microscope that will be understandable to the broad range of scientific fields that presently exploit, or wish to begin exploiting, this powerful technology. With this in mind, we have developed a guide to aid in the design of a multiphoton microscope. We discuss source selection, optical management of dispersion, image-relay systems with scan optics, objective-lens selection, single-element light-collection theory, photon-counting detection, image rendering, and finally, an illustrated guide for building an example microscope.
多光子显微镜已成为广泛学科中用于研究微观结构和功能的普遍工具。因此，本文旨在为多光子显微镜的构建和性能评估提供全面的资源，以便目前正在使用或希望开始使用这项强大技术的广大科学领域能够理解。考虑到这一点，我们编写了一本指南来辅助多光子显微镜的设计。我们讨论了光源选择、色散的光学管理、带有扫描光学器件的图像中继系统、物镜选择、单元素光收集理论、光子计数检测、图像渲染，最后还提供了一个构建示例显微镜的图文指南。
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