基于空间光调制器的寻址式多区域多焦点多光子显微镜。
Addressable multiregional and multifocal multiphoton microscopy based on a spatial light modulator.
出版信息
J Biomed Opt. 2012 Mar;17(3):030505. doi: 10.1117/1.JBO.17.3.030505.
Abstract
Through a combination of a deflective phase-only diffractive spatial light modulator (SLM) and galvo scanners, an addressable multiregional and multifocal multiphoton microscope (AM-MMM) is developed. The SLM shapes an incoming mode-locked, near-infrared Ti:sapphire laser beam into multiple beamlet arrays with addressable shapes and sizes that match the regions of interest on the sample. Compared with conventional multifocal multiphoton microscope (MMM), AM-MMM achieves the effective use of the laser power with an increase of imaging rate and a decrease of photodamage without sacrifice of resolution.
摘要
通过组合使用具有偏折相位的纯相位衍射型空间光调制器 (SLM) 和振镜扫描器，开发了一种可寻址的多区域多焦点多光子显微镜 (AM-MMM)。SLM 将入射的锁模近红外钛宝石激光光束整形为具有可寻址形状和大小的多个射束阵列，这些形状和大小与样品上的感兴趣区域匹配。与传统的多焦点多光子显微镜 (MMM) 相比，AM-MMM 实现了激光功率的有效利用，同时提高了成像速度，降低了光损伤，而不会牺牲分辨率。
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