表皮生长因子（EGF）对分离的大鼠肝细胞中ATP柠檬酸裂解酶活性的刺激作用具有年龄依赖性。
Epidermal growth factor (EGF) stimulation of ATP citrate lyase activity in isolated rat hepatocytes is age dependent.
作者信息
Emmerson K, Roehrig K
机构信息
Department of Food Science and Technology, The Ohio State University, Columbus 43210.
出版信息
Comp Biochem Physiol B. 1992 Nov;103(3):663-7. doi: 10.1016/0305-0491(92)90387-7.
PMID:1458840
Abstract
- The effect of epidermal growth factor (EGF) on ATP citrate lyase activity was determined in freshly isolated hepatocytes from rats of different ages as a function of incubation time, EGF concentration and hepatocyte density. 2. The activity of this enzyme was responsive to both dose and time of incubation of EGF with a two-fold increase in ATP citrate lyase activity and a half-maximal effect between 10(-12) and 10(-11) M. 3. EGF effects were detectable by 5 min. 4. The age of the rats had a strong effect on the magnitude of the EGF effect with ATP citrate lyase activity in younger (8 weeks) rats being more responsive than in older (14 weeks) rats.
摘要
- 测定了表皮生长因子（EGF）对不同年龄大鼠新鲜分离肝细胞中ATP柠檬酸裂解酶活性的影响，该影响是孵育时间、EGF浓度和肝细胞密度的函数。2. 该酶的活性对EGF孵育的剂量和时间均有反应，ATP柠檬酸裂解酶活性增加两倍，在10（-12）至10（-11）M之间达到半数最大效应。3. 5分钟时可检测到EGF的作用。4. 大鼠年龄对EGF作用的幅度有强烈影响，较年轻（8周）大鼠的ATP柠檬酸裂解酶活性比年长（14周）大鼠对EGF更敏感。
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