淡水龟锦龟体内假定的载脂蛋白B-100：雌激素和孕酮的影响。

Putative apolipoprotein B-100 in the freshwater turtle Chrysemys picta: effects of estrogen and progesterone.

Perez L E, Williams D, Callard I P

Department of Biology, Boston University, MA 02215.

Comp Biochem Physiol B. 1992 Nov;103(3):707-13. doi: 10.1016/0305-0491(92)90394-7.

Abstract

The isolation and purification of a putative apolipoprotein B-100 in the plasma of the freshwater turtle Chrysemys picta is described. 2. The protein was purified through differential ultracentrifugation and subsequent Sepharose 6B column chromatography. 3. The molecular weight of the protein determined by electrophoresis was approximately 350 kDa. 4. An antibody to chicken apolipoprotein B-100 specifically recognizes this 350 kDa protein in Western blots, suggesting its identity with apolipoprotein B-100. 5. An antibody to the putative Chrysemys apolipoprotein B-100-like protein was developed and used in an ELISA to quantitate protein levels in plasma. 6. Acute estrogen treatment increased levels of apolipoprotein B-100 (7.64 +/- 0.79 mg/ml plasma) over that of control animals (5.07 +/- 1.74 mg/ml plasma). 7. In contrast, chronic estrogen treatment reduced apolipoprotein B-100 significantly to 2.94 +/- 0.53 mg/ml plasma (P < 0.05).

摘要

描述了从淡水龟锦龟血浆中分离和纯化一种假定的载脂蛋白B-100的过程。2. 该蛋白质通过差速超速离心和随后的琼脂糖6B柱色谱法进行纯化。3. 通过电泳测定该蛋白质的分子量约为350 kDa。4. 抗鸡载脂蛋白B-100抗体在蛋白质免疫印迹中能特异性识别这种350 kDa的蛋白质，表明其与载脂蛋白B-100相同。5. 制备了针对假定的锦龟载脂蛋白B-100样蛋白的抗体，并用于酶联免疫吸附测定（ELISA）以定量血浆中的蛋白质水平。6. 急性雌激素处理使载脂蛋白B-100水平（7.64±0.79 mg/ml血浆）高于对照动物（5.07±1.74 mg/ml血浆）。7. 相比之下，慢性雌激素处理使载脂蛋白B-100显著降低至2.94±0.53 mg/ml血浆（P<0.05）。