Jin Y Z, Ding L, Shen Z F, Cai R M, Xu L M, Yang J K, Jin X Q, Lu W Q, Xu J F
Institute of Radiation Medicine, Shanghai Medical University.
Chin Med J (Engl). 1992 Aug;105(8):647-50.
An inhibitor of glutathione biosynthesis, buthionine sulphoximine (BSO), was used to deplete the endogenous thiols in mammalian cells in vitro. In this study, the cytotoxicity of BSO and BSO combined with the hypoxic cell radiosensitizer misonidazole (MISO) was investigated. Both aerobic and hypoxic cytotoxicity of MISO was found to be increased. The concentration of BSO required to reduce the colony forming ability to 50% (Cc) for the chronic cytotoxicity on V79 cells was 0.03 mmol/L under aerobic condition, while the Cc for the acute cytotoxicity on V79 cells under hypoxic and aerobic conditions was 0.4 and 0.5 mmol/L. The growth inhibition rate of human tumor cells K562 and SGC-7901 by BSO was 6.89-26.06% and 12.01-55.69%, respectively. Enhanced cytotoxicity activity was observed when BSO was used in combination with cis-dichlorodiamino Pt(II) or 5-fluorouracil.
谷胱甘肽生物合成抑制剂丁硫氨酸亚砜胺（BSO）被用于在体外耗尽哺乳动物细胞中的内源性硫醇。在本研究中，研究了BSO以及BSO与乏氧细胞放射增敏剂米索硝唑（MISO）联合使用时的细胞毒性。结果发现，MISO的需氧和乏氧细胞毒性均有所增加。在有氧条件下，将V79细胞的集落形成能力降低50%（Cc）所需的慢性细胞毒性的BSO浓度为0.03 mmol/L，而在乏氧和有氧条件下对V79细胞急性细胞毒性的Cc分别为0.4和0.5 mmol/L。BSO对人肿瘤细胞K562和SGC - 7901的生长抑制率分别为6.89 - 26.06%和12.01 - 55.69%。当BSO与顺二氯二氨基铂（II）或5 - 氟尿嘧啶联合使用时，观察到细胞毒性活性增强。
