Matsunaga K, Mashiba H
Division of Immunology, National Kyushu Cancer Center, Fukuoka, Japan.
Eur Cytokine Netw. 1992 May-Jun;3(3):307-11.
The effect of buthionine sulfoximine (BSO), an inhibitor of glutathione biosynthesis, in combined use with a nitrosourea derivative, ACNU, on the cytostatic effect of recombinant human lymphotoxin (rhLT) was studied in vitro. The simultaneous addition of 0.02 mM or 0.5 mM BSO and rhLT slightly augmented the inhibition of Meth A tumor cell proliferation. Similar tendency was observed when the target cells were treated with 0.02 mM or 0.5 mM BSO for 24 hr prior to the addition of rhLT. A marked augmentation of the antiproliferative effect was obtained when the target cells were treated in vitro with 0.005 mM or 0.02 mM BSO prior to the addition of 0.02 mM or 0.1 mM BSO and rhLT. The addition of ACNU simultaneously with rhLT to BSO-treated cells also augmented the antiproliferative effect. These results suggest that the glutathione redox cycle is closely related to the mechanism of LT-induced cytotoxicity.
在体外研究了谷胱甘肽生物合成抑制剂丁硫氨酸亚砜亚胺（BSO）与亚硝基脲衍生物ACNU联合使用对重组人淋巴毒素（rhLT）细胞抑制作用的影响。同时添加0.02 mM或0.5 mM BSO和rhLT可略微增强对Meth A肿瘤细胞增殖的抑制作用。当在添加rhLT之前用0.02 mM或0.5 mM BSO处理靶细胞24小时时，观察到类似的趋势。当在添加0.02 mM或0.1 mM BSO和rhLT之前用0.005 mM或0.02 mM BSO体外处理靶细胞时，可获得抗增殖作用的显著增强。在将ACNU与rhLT同时添加到经BSO处理的细胞中时，也增强了抗增殖作用。这些结果表明，谷胱甘肽氧化还原循环与LT诱导的细胞毒性机制密切相关。
