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Manual sequencing using pulsed field.

作者信息

Turmel C, Brassard E, Noolandi J

机构信息

Xerox Research Centre of Canada, Mississauga, Ontario.

出版信息

Electrophoresis. 1992 Sep-Oct;13(9-10):620-2. doi: 10.1002/elps.11501301126.

DOI:10.1002/elps.11501301126
PMID:1459077
Abstract

The use of pulsed fields in manual sequencing opens up the compression zone found with a DC field and extends the range of resolution from a few hundred bases to several thousand bases. The band inversion problem is overcome with the proper pulsing conditions, and the bands are sharper than for the DC field case. Accurate visual reading is possible up to about 800-900 bases. The method is compatible with automation techniques, since the band spectrum is stretched continuously during migration, and the smaller fragments are run off the gel.

摘要

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引用本文的文献

1
Sequencing using pulsed field and image reconstruction.使用脉冲场和图像重建进行测序。
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10101-4. doi: 10.1073/pnas.90.21.10101.