使用脉冲场和图像重建进行测序。
Sequencing using pulsed field and image reconstruction.
作者信息
Noolandi J, Forsyth R, Shi A C
机构信息
Xerox Research Centre of Canada, Mississauga, Ontario.
出版信息
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10101-4. doi: 10.1073/pnas.90.21.10101.
The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.
在标准手动测序设置中使用脉冲场，与直流场在单一凝胶上分离300 - 400个碱基相比，可分离超过2 kb的片段。然而，在最佳条件下，从曝光于脉冲场凝胶的胶片上目视读取超过800 - 900个碱基的序列是不可能的。使用图像重建和增强技术可以读取超过1 kb的M13mp18序列，并且在超过2 kb处可以识别出单个条带。
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