铜绿假单胞菌PAO染色体的PacI/SwaI酶切图谱。

A PacI/SwaI map of the Pseudomonas aeruginosa PAO chromosome.

作者信息

Römling U, Tümmler B

机构信息

Abteilung Biophysikalische Chemie, Medizinische Hochschule, Hannover, Germany.

出版信息

Electrophoresis. 1992 Sep-Oct;13(9-10):649-51. doi: 10.1002/elps.11501301135.

DOI:10.1002/elps.11501301135

PMID:1459083

Abstract

The PacI/SwaI macrorestriction map of the 5.9 Mb Pseudomonas aeruginosa PAO (DSM 1707) genome was constructed by fragment size comparisons of complete single and double digests and hybridization with probes of known genomic localization. P. aeruginosa PAO contains five recognition sites for PacI and six sites for SwaI. An integrated DpnI/PacI/SpeI/SwaI map was obtained by fragment pattern and Southern blot hybridization analyses of all combinations of double digestions and by two-dimensional pulsed-field gel electrophoresis mapping protocols.

摘要

通过对完整单酶切和双酶切片段大小进行比较，并与已知基因组定位的探针杂交，构建了5.9 Mb铜绿假单胞菌PAO（DSM 1707）基因组的PacI/SwaI宏观限制性图谱。铜绿假单胞菌PAO含有5个PacI识别位点和6个SwaI识别位点。通过对所有双酶切组合进行片段模式分析和Southern杂交分析，以及采用二维脉冲场凝胶电泳图谱分析方法，获得了整合的DpnI/PacI/SpeI/SwaI图谱。