Ratnaningsih E, Dharmsthiti S, Krishnapillai V, Morgan A, Sinclair M, Holloway B W
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
J Gen Microbiol. 1990 Dec;136(12):2351-7. doi: 10.1099/00221287-136-12-2351.
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.
通过脉冲场凝胶电泳和Southern杂交技术，利用携带已知基因的基因组文库中的黏粒克隆，构建了铜绿假单胞菌PAO的物理图谱和遗传图谱。在5862 kb的基因组上共定位了37个SpeI限制性片段，通过与SpeI连接片段文库中的克隆以及部分SpeI消化获得的片段进行杂交，证明了片段的连续性，这些片段均来自铜绿假单胞菌PAO染色体。
