在半透明带试验中，新鲜的和经冷冻解冻的人类精子与透明带的结合模式相似。

Fresh and frozen-thawed human sperm bind in a similar pattern to the zona pellucida in the hemizona assay.

作者信息

Gamzu R, Yogev L, Yavetz H, Homonnai Z T, Hiss Y, Paz G

机构信息

Institute for the Study of Fertility, Serlin Maternity Hospital, Tel Aviv, Israel.

出版信息

Fertil Steril. 1992 Dec;58(6):1254-6. doi: 10.1016/s0015-0282(16)55582-4.

DOI:10.1016/s0015-0282(16)55582-4

PMID:1459282

Abstract

This study evaluated the impact of sperm cryopreservation on sperm quality. The HZA was used to test the binding capacity of fresh as opposed to frozen-thawed sperm from 12 donors. Fresh and frozen-thawed sperm motility was 47% +/- 1.5% and 24% +/- 3.8% (mean +/- SE), respectively. However, the number of sperm cells attached to the hemizonae was 75 +/- 12.0 and 74 +/- 11.9, respectively. We conclude that cryopreservation results in a reduced number of motile sperm cells but does not adversely affect the ability of rescued sperm cells to bind to the ZP. The study also supports the use of frozen-thawed rather than fresh donor sperm for control in the HZA procedure.

摘要

本研究评估了精子冷冻保存对精子质量的影响。采用半透明带分析法（HZA）检测了12名供体的新鲜精子与冻融精子的结合能力。新鲜精子和冻融精子的活力分别为47%±1.5%和24%±3.8%（平均值±标准误）。然而，附着在半透明带上的精子细胞数量分别为75±12.0和74±11.9。我们得出结论，冷冻保存会导致活动精子细胞数量减少，但不会对获救精子细胞与透明带（ZP）结合的能力产生不利影响。该研究还支持在HZA程序中使用冻融而非新鲜供体精子作为对照。