衰老、透明质酸酶去除卵丘以及显微注射均不影响人类卵母细胞的精子结合潜能。
Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.
作者信息
Barros A, Sousa M, Silva J, Almeida V, Rocha E
机构信息
Laboratory of Medical Genetics, Faculty of Medicine, University of Porto, Portugal.
出版信息
J Assist Reprod Genet. 1997 Feb;14(2):97-101. doi: 10.1007/BF02765778.
PURPOSE
We studied the influence of aging, hyaluronidase removal of the cumulus, and microinjection on the sperm binding potential of human oocytes under intact zona assay conditions to determine the safe use of unstored aged unfertilized ICSI oocytes on zona binding tests. Results were also compared with those for aged IVF oocytes under the same conditions.
METHODS
To avoid the large variation in sperm binding scores, we compared the ratios of the number of sperm bound to nontreated versus to treated oocytes. Treated oocytes were those whose zonae were exposed to hyaluronidase, an agent that decreases sperm binding. Experimental groups were fresh oocytes, experimentally aged fresh oocytes, fresh oocytes whose cumulus was removed either mechanically or with hyaluronidase, aged ICSI oocytes, and aged IVF oocytes.
RESULTS
Statistical analysis within and between groups showed that aging, previous insemination, cumulus removal by hyaluronidase, and microinjection did not affect the sperm binding potential of human oocytes.
CONCLUSIONS
As the zona binding ability of fresh and unfertilized aged ICSI oocytes is similar, they can be used safely in zona assays.
目的
我们研究了衰老、用透明质酸酶去除卵丘以及显微注射对完整透明带检测条件下人类卵母细胞精子结合潜能的影响，以确定未储存的衰老未受精卵胞浆内单精子注射（ICSI）卵母细胞在透明带结合试验中的安全使用情况。同时，将结果与相同条件下的衰老体外受精（IVF）卵母细胞进行比较。
方法
为避免精子结合评分的大幅波动，我们比较了未处理卵母细胞与处理后卵母细胞的精子结合数量之比。处理后的卵母细胞是指其透明带暴露于透明质酸酶的卵母细胞，透明质酸酶是一种会降低精子结合能力的物质。实验组包括新鲜卵母细胞、实验性老化的新鲜卵母细胞、通过机械方法或使用透明质酸酶去除卵丘的新鲜卵母细胞、老化的ICSI卵母细胞以及老化的IVF卵母细胞。
结果
组内和组间的统计分析表明，衰老、先前的授精、用透明质酸酶去除卵丘以及显微注射均不影响人类卵母细胞的精子结合潜能。
结论
由于新鲜和未受精的老化ICSI卵母细胞的透明带结合能力相似，它们可安全用于透明带检测。
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