单亲细胞融合：一种使四膜虫微核突变在早熟性成熟时纯合到大核表达的新方法。
Uniparental cytogamy: a novel method for bringing micronuclear mutations of Tetrahymena into homozygous macronuclear expression with precocious sexual maturity.
作者信息
Cole E S, Bruns P J
机构信息
Department of Genetics and Development, Cornell University, Ithaca, New York 14853.
出版信息
Genetics. 1992 Dec;132(4):1017-31. doi: 10.1093/genetics/132.4.1017.
Abstract
A new method of inducing self-fertilization, uniparental cytogamy, yields homozygous germinal and somatic genotypes in the ciliate Tetrahymena thermophila. Progeny are highly fertile and show a marked tendency for precocious sexual maturity. This method is highly effective in protocols designed to generate and express nonlethal dominant or recessive mutations.
摘要
一种诱导自体受精的新方法——单亲细胞融合，可在嗜热四膜虫中产生纯合的生殖型和体细胞型基因型。后代具有高度的繁殖力，并且表现出明显的早熟性成熟倾向。在旨在产生和表达非致死性显性或隐性突变的实验方案中，这种方法非常有效。
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