Cassidy-Hanley Donna M
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.
Methods Cell Biol. 2012;109:237-76. doi: 10.1016/B978-0-12-385967-9.00008-6.
The ciliated protozoan Tetrahymena thermophila has been an important model system for biological research for many years. During that time, a variety of useful strains, including highly inbred stocks, a collection of diverse mutant strains, and wild cultivars from a variety of geographical locations have been identified. In addition, thanks to the efforts of many different laboratories, optimal conditions for growth, maintenance, and storage of Tetrahymena have been worked out. To facilitate the efficient use of Tetrahymena, especially by those new to the system, this chapter presents a brief description of many available Tetrahymena strains and lists possible resources for obtaining viable cultures of T. thermophila and other Tetrahymena species. Descriptions of commonly used media, methods for cell culture and maintenance, and protocols for short- and long-term storage are also presented.
多年来，嗜热四膜虫这种纤毛原生动物一直是生物学研究的重要模型系统。在此期间，已鉴定出多种有用的菌株，包括高度近交系、各种突变菌株的集合以及来自不同地理位置的野生品种。此外，由于许多不同实验室的努力，已经确定了嗜热四膜虫生长、维持和保存的最佳条件。为了便于高效利用嗜热四膜虫，特别是对于该系统的新手，本章简要介绍了许多现有的嗜热四膜虫菌株，并列出了获取嗜热四膜虫和其他四膜虫物种活培养物的可能资源。还介绍了常用培养基的描述、细胞培养和维持方法以及短期和长期保存方案。
