Selden Clare, Chalmers Sherri-Ann, Jones Catherine, Standish Richard, Quaglia Alberto, Rolando Nancy, Burroughs Andrew K, Rolles Keith, Dhillon Amar, Hodgson Humphrey J F
Centre for Hepatology, Department of Medicine, Royal Free and University College Medical School, Hampstead, London, United Kingdom.
Stem Cells. 2003;21(6):624-31. doi: 10.1634/stemcells.21-6-624.
The liver in subacute hepatic failure may become enriched for hepatic progenitor cells. Liver tissue from such a patient was collagenase digested and, from the nonparenchymal cell fraction, epithelioid colonies were developed. Albumin and alpha-1-antitrypsin (AAT) were secreted for greater than 120 days from these colonies. Reverse transcription-polymerase chain reaction showed expression of markers of both hepatocyte and biliary epithelial phenotypes (cytokeratins 7, 18, and 19, albumin and AAT, hepatocyte growth factor receptor, transforming growth factor beta receptor type II, gamma-glutamyl transpeptidase, biliary glycoprotein). The cell cycle regulator p21 was also expressed. The POU domain transcription factor octamer-binding protein 4 was present in these cells, but not in RNA or cDNA prepared from adult human liver. These markers were maintained even after 165 days culture. Proliferating epithelial-like cells with combined hepatocyte- and biliary-epithelial-specific functional markers and a stem cell marker can be isolated from the nonparenchymal fraction of liver cells in subacute hepatic failure.
在亚急性肝衰竭中，肝脏可能会富集肝祖细胞。对一名此类患者的肝脏组织进行胶原酶消化处理，并从非实质细胞部分培养出上皮样集落。这些集落分泌白蛋白和α-1-抗胰蛋白酶（AAT）超过120天。逆转录-聚合酶链反应显示同时表达肝细胞和胆管上皮细胞表型的标志物（细胞角蛋白7、18和19、白蛋白和AAT、肝细胞生长因子受体、转化生长因子β II型受体、γ-谷氨酰转肽酶、胆管糖蛋白）。细胞周期调节因子p21也有表达。POU结构域转录因子八聚体结合蛋白4存在于这些细胞中，但不存在于从成人肝脏制备的RNA或cDNA中。即使在培养165天后，这些标志物仍能维持。具有肝细胞和胆管上皮细胞特异性功能标志物以及干细胞标志物的增殖性上皮样细胞可从亚急性肝衰竭患者的肝细胞非实质部分分离得到。
