Jagota S K
Department of Biochemistry, Panjab University, Chandigarh.
Indian J Med Res. 1992 Oct;96:275-8.
In vitro inhibitory effect of dimethylsulfoxide (DMSO) on acetylcholinesterase of erythrocyte membranes and synaptosomes of mice was observed using acetylthiocholine as substrate. DMSO inhibited the enzyme from both sources and the effect was concentration dependent. It produced an inhibition of 26 to 28 per cent at a concentration of 0.13 mM and 92 to 95 per cent at a higher concentration of 1.91 mM. The Km of bound and solubilized enzyme of the synaptosomes was 0.2 and 0.15 mM while that of erythrocyte membranes 0.5 and 0.33 mM respectively. The Vmax was 0.4 and 0.4 (for synaptosomes) and 0.67 and 0.59 (for erythrocyte membranes) Mmoles/mg protein/min, respectively for bound and solubilized forms. The Ki of solubilized enzyme of synaptosomes was 0.11 mM. Kinetic studies showed that the inhibition was competitive in nature.
以乙酰硫代胆碱为底物，观察了二甲基亚砜（DMSO）对小鼠红细胞膜和突触体乙酰胆碱酯酶的体外抑制作用。DMSO抑制了这两种来源的酶，且该作用具有浓度依赖性。在浓度为0.13 mM时，抑制率为26%至28%，在浓度较高的1.91 mM时，抑制率为92%至95%。突触体结合型和可溶型酶的Km分别为0.2和0.15 mM，而红细胞膜的Km分别为0.5和0.33 mM。结合型和可溶型的Vmax分别为0.4和0.4（突触体）以及0.67和0.59（红细胞膜）微摩尔/毫克蛋白/分钟。突触体可溶型酶的Ki为0.11 mM。动力学研究表明，该抑制作用本质上是竞争性的。
