Langa S, Fernández A, Martín R, Reviriego C, Marín M L, Fernández L, Rodríguez J M
Departamento de Nutrición y Bromatología III, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Int J Food Microbiol. 2003 Dec 1;88(2-3):197-200. doi: 10.1016/s0168-1605(03)00180-6.
Variations in length and sequence of the 16S/23S spacer region of Enterococcus faecium provided the basis for development of simple PCR and dot-blot hybridisation assays that enabled the differentiation of potentially probiotic Enterococcus faecium strains from Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Such assays may be useful for differentiation of yoghurt starter cultures and enterococcal strains when they are simultaneously present in probiotic food products.
粪肠球菌16S/23S间隔区长度和序列的变化为开发简单的PCR和斑点杂交检测方法提供了基础，这些检测方法能够区分潜在的益生菌粪肠球菌菌株与德氏乳杆菌保加利亚亚种和嗜热链球菌。当酸奶发酵剂培养物和肠球菌菌株同时存在于益生菌食品中时，此类检测方法可能有助于区分它们。
