[通过16S rRNA的PCR扩增和序列分析鉴定嗜酸乳杆菌和嗜热链球菌]
[Identification of Lactobacillus and Streptococcus thermophilus by PCR amplification and sequence analysis of 16S rRNA].
作者信息
Dong Yinping, Cui Shenghui, Li Fengqin, Yu Hongxia
机构信息
Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China.
出版信息
Wei Sheng Yan Jiu. 2010 Jul;39(4):454-8, 465.
OBJECTIVE
To develop a PCR method for identifying the 16S rRNA of Lactobacillus and Streptococcus thermophilus at the species level.
METHODS
Optimizing the method for DNA extraction and the conditions for PCR amplification. Joining the PCR amplification products from 16S rRNA to plasmid puc18-T and detecting the sequence.
RESULTS
All 50 isolates recovered from yoghourt products were characterized by 16S rRNA sequence analysis and 7 groups were identified as L. bulgaricus (24 strains), S. thermophilus (12 strains), L. acidophilus (7 strains), L. casei (3 strains), L. delbrueckii (2 strains), L. fermentum (1 strain) and S. lutetiensis (1 strain).
CONCLUSION
16S rRNA PCR method developed in this research is a sensitive and reliable method for the identification of both Lactobacillus and Streptococcus thermophilus.
目的
建立一种在种水平上鉴定乳酸杆菌和嗜热链球菌16S rRNA的PCR方法。
方法
优化DNA提取方法和PCR扩增条件。将16S rRNA的PCR扩增产物连接到质粒puc18-T上并检测序列。
结果
从酸奶产品中回收的所有50株分离株通过16S rRNA序列分析进行鉴定，7组被鉴定为保加利亚乳杆菌（24株）、嗜热链球菌（12株）、嗜酸乳杆菌（7株）、干酪乳杆菌（3株）、德氏乳杆菌（2株）、发酵乳杆菌（1株）和路氏链球菌（1株）。
结论
本研究建立的16S rRNA PCR方法是一种用于鉴定乳酸杆菌和嗜热链球菌的灵敏且可靠的方法。
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