Keski-Saari Sarita, Julkunen-Tiitto Riita
Natural Product Research Laboratories, Department of Biology, University of Joensuu, FIN-80101 Joensuu, Finland.
Tree Physiol. 2003 Dec;23(17):1201-8. doi: 10.1093/treephys/23.17.1201.
Phenolic compounds, identified by high performance liquid chromatography (HPLC), and growth were investigated in the first true leaves, cotyledons, stems and roots of 2.5-week-old seedlings of mountain birch (Betula pubescens subsp. czerepanovii (Orlova) Hämetahti) grown with 1.5, 15 and 50 ppm phosphorus (P). Phenolic compounds in seeds and unfertilized 6-day-old seedlings were also studied. Seeds contained few phenolics, but had an abundance of condensed tannins. There were fewer HPLC phenolic compounds in 6-day-old seedlings than in 2.5-week-old seedlings. In 2.5-week-old seedlings, growth of the first true leaves, but not of other plant parts, was affected by P regime. In the first true leaves and stems, the concentration of condensed tannins was significantly affected by P, being higher at 1.5 ppm than at higher concentrations. The concentration of total HPLC phenolics was significantly affected by P only in stems.
通过高效液相色谱法（HPLC）鉴定的酚类化合物以及生长情况，在分别以1.5、15和50 ppm磷（P）培养的2.5周龄欧洲山桦（Betula pubescens subsp. czerepanovii (Orlova) Hämetahti）幼苗的第一片真叶、子叶、茎和根中进行了研究。还对种子和未施肥的6日龄幼苗中的酚类化合物进行了研究。种子中酚类物质含量很少，但富含缩合单宁。6日龄幼苗中的HPLC酚类化合物比2.5周龄幼苗中的少。在2.5周龄幼苗中，第一片真叶的生长受到磷处理的影响，但其他植物部位不受影响。在第一片真叶和茎中，缩合单宁的浓度受磷的显著影响，在1.5 ppm时高于较高浓度时。仅在茎中，总HPLC酚类化合物的浓度受磷的显著影响。
