Ossipov V, Loponen J, Haukioja E, Pihlaja K
Department of Chemistry, University of Turku, Finland.
J Chromatogr A. 1999 Dec 24;864(2):283-91. doi: 10.1016/s0021-9673(99)01036-5.
A high-performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS) method, assisted by diode array detection, for the characterisation of individual hydrolysable tannins in birch leaves was developed. With the method, it was found that birch (Betula pubescens) leaves contained an exceptionally complex mixture of hydrolysable tannins; 14 gallotannins and 20 ellagitannins were identified. The developed HPLC-ESI-MS method allows the qualitative and quantitative determination of individual gallotannins and ellagitannins directly from crude birch leaf extract. This is important in studying ecological functions of these phenolic compounds, especially their role in the resistance of birch leaves against insects.
建立了一种采用二极管阵列检测辅助的高效液相色谱-电喷雾电离质谱（HPLC-ESI-MS）方法，用于表征桦树叶中各水解单宁。通过该方法发现，桦树（毛桦）叶中含有异常复杂的水解单宁混合物；鉴定出14种没食子单宁和20种鞣花单宁。所建立的HPLC-ESI-MS方法可直接从桦树叶粗提物中对各没食子单宁和鞣花单宁进行定性和定量测定。这对于研究这些酚类化合物的生态功能，尤其是它们在桦树叶抗虫性中的作用具有重要意义。
